Grupos de investigación

Publicaciones / Publications

Targeting Aspergillus fumigatus Crf Transglycosylases With Neutralizing Antibody Is Relevant but Not Sufficient to Erase Fungal Burden in a Neutropenic Rat Model.

Chauvin D, Hust M, Schütte M, Chesnay A, Parent C, Moreira GMSG, Arroyo J, Sanz AB, Pugnière M, Martineau P, Chandenier J, Heuzé-Vourc'h N, Desoubeaux G. Front Microbiol. 2019 Mar 26;10:600


Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases.

Fang W, Sanz AB, Bartual SG, Wang B, Ferenbach AT, Farkaš V, Hurtado-Guerrero R, Arroyo J, van Aalten DMF. Nat Commun. 2019 Apr 10;10(1):1669.


Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress.

García R, Pulido V, Orellana-Muñoz S, Nombela C, Vázquez de Aldana CR, Rodríguez-Peña JM, Arroyo J. Sci Rep. 2019 Feb 28;9(1):3186


Slt2 MAPK association with chromatin is required for transcriptional activation of Rlm1 dependent genes upon cell wall stress.

Sanz AB, García R, Rodríguez-Peña JM, Nombela C, Arroyo J. Biochim Biophys Acta Gene Regul Mech. 2018 Nov;1861(11):1029-1039 


The CWI Pathway: Regulation of the Transcriptional Adaptive Response to Cell Wall Stress in Yeast.

Sanz AB, García R, Rodríguez-Peña JM, Arroyo J. J Fungi (Basel). 2017 Dec 21;4(1). pii: E1 Review.


A novel connection between the Cell Wall Integrity and the PKA pathways regulates cell wall stress response in yeast.

García R, Bravo E, Diez-Muñiz S, Nombela C, Rodríguez-Peña JM, Arroyo J. Sci Rep. 2017 Jul 18;7(1):5703.


Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling.

Degani G, Ragni E, Botias P, Ravasio D, Calderon J, Pianezzola E, Rodriguez-Peña JM, Vanoni MA, Arroyo J, Fonzi WA, Popolo L. BMC Genomics. 2016 Jul 2;17(1):482


Strengthening the fungal cell wall through chitin-glucan cross-links: effects on morphogenesis and cell integrity.

Arroyo J, Farkaš V, Sanz AB, Cabib E. Cell Microbiol. 2016 Sep;18(9):1239-50. doi: 10.1111/cmi.12615


Cooperation between SAGA and SWI/SNF complexes is required for efficient transcriptional responses regulated by the yeast MAPK Slt2.

Sanz AB, García R, Rodríguez-Peña JM, Nombela C, Arroyo J. Nucleic Acids Res. 2016 Apr 25. pii: gkw324


Rlm1 mediates a positive autoregulatory transcriptional feedback essential for Slt2 MAPK dependent gene expression.

García R, Sanz AB, Rodríguez-Peña JM, Nombela C, Arroyo J. J. Cell Science. 2016 Apr 15;129(8):1649-60


•Genomic profiling of fungal cell wall-interfering compounds: identification of a common gene signature.

García R, Botet J, Rodríguez-Peña JM, Bermejo C, Ribas JC, Revuelta JL, Nombela C, Arroyo J. BMC Genomics. 2015 Sep 5;16(1):683


Structural and functional analysis of yeast Crh1 and Crh2 transglycosylases.

Blanco, N, Hurtado-Guerrero, R., Sanz, A.B., Rodríguez-Peña, JM, Farkaš, V, Arroyo, J. FEBS Journal. 2015.282: 715-731.


A novel fluorescence assay and catalytic properties of Crh1 and Crh2 yeast cell wall transglycosylases.

Mazáň M, Blanco N, Kováčová K, Firáková Z, Rehulka P, Farkaš V, Arroyo J. Biochem J. 2013 Nov 1;455(3):307-18


Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2.

Rodríguez-Peña JM, Díez-Muñiz S, Bermejo C, Nombela C, Arroyo J. FEBS Lett. 2013 Oct 4. doi:pii: S0014-5793(13)00725-4


How carbohydrates sculpt cells: chemical control of morphogenesis in the yeast cell wall.

Cabib E, Arroyo J. Nat Rev Microbiol. 2013 Sep;11(9):648-55


Crosslinks in the cell wall of budding yeast control morphogenesis at the mother-bud neck.

Blanco N, Reidy M, Arroyo J, Cabib E. J Cell Sci. 2012 Dec 1;125(Pt 23):5781-9


Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway.

Sanz AB, García R, Rodríguez-Peña JM, Díez-Muñiz S, Nombela C, Peterson CL, Arroyo J. Mol Biol Cell. 2012 Jul;23(14):2805-17


Presence of a large β(1-3)glucan linked to chitin at the Saccharomyces cerevisiae mother-bud neck suggests involvement in localized growth control.

Cabib E, Blanco N, Arroyo J. Eukaryot Cell. 2012 Apr;11(4):388-400


Sensing of mammalian IL-17A regulates fungal adaptation and virulence.

Zelante T, Iannitti RG, De Luca A, Arroyo J, Blanco N, Servillo G, Sanglard D, Reichard U, Palmer GE, Latgè JP, Puccetti P, Romani L. Nat Commun. 2012 Feb 21;3:683


Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: novel insights into diverse MAPK outcomes.

Arias P, Díez-Muñiz S, García R, Nombela C, Rodríguez-Peña JM, Arroyo J. BMC Genomics. 2011 Aug 2;12:390


Expression, stability, and replacement of glucan-remodeling enzymes during developmental transitions in Saccharomyces cerevisiae.

Rolli E, Ragni E, de Medina-Redondo M, Arroyo J, de Aldana CR, Popolo L. Mol Biol Cell. 2011 May;22(9):1585-98


Functional and genomic analyses of blocked protein O-mannosylation in baker's yeast.

Arroyo J, Hutzler J, Bermejo C, Ragni E, García-Cantalejo J, Botías P, Piberger H, Schott A, Sanz AB, Strahl S. Mol Microbiol. 2011 Mar;79(6):1529-46


The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections.

Ragni E, Piberger H, Neupert C, García-Cantalejo J, Popolo L, Arroyo J, Aebi M, Strahl S. BMC Genomics. 2011 Feb 14;12:107


Characterization of sensor-specific stress response by transcriptional profiling of wsc1 and mid2 deletion strains and chimeric sensors in Saccharomyces cerevisiae.

Bermejo C, García R, Straede A, Rodríguez-Peña JM, Nombela C, Heinisch JJ, Arroyo J. OMICS. 2010 Dec;14(6):679-88.


The high-osmolarity glycerol (HOG) and cell wall integrity (CWI) signalling pathways interplay: a yeast dialogue between MAPK routes.

Rodríguez-Peña JM, García R, Nombela C, Arroyo J. Yeast. 2010 Aug;27(8):495-502.


GAS3, a developmentally regulated gene, encodes a highly mannosylated and inactive protein of the Gas family of Saccharomyces cerevisiae.

Rolli E, Ragni E, Rodriguez-Peña JM, Arroyo J, Popolo L. Yeast. 2010 Aug;27(8):597-610


MARQ: an online tool to mine GEO for experiments with similar or opposite gene expression signatures.

Vazquez M, Nogales-Cadenas R, Arroyo J, Botías P, García R, Carazo JM, Tirado F, Pascual-Montano A, Carmona-Saez P. Nucleic Acids Res. 2010 Jul;38(Web Server issue):W228-32


Mtl1 is required to activate general stress response through Tor1 and Ras2 inhibition under conditions of glucose starvation and oxidative stress.

Petkova MI, Pujol-Carrion N, Arroyo J, García-Cantalejo J, Angeles de la Torre-Ruiz M. J Biol Chem. 2010 Jun 18;285(25):19521-31


Immune responses of human immature dendritic cells can be modulated by the recombinant Aspergillus fumigatus antigen Aspf1.

Ok M, Latgé JP, Baeuerlein C, Ebel F, Mezger M, Topp M, Kurzai O, Killian D, Kapp M, Grigoleit GU, Sennefelder H, Arroyo J, Einsele H, Loeffler J. Clin Vaccine Immunol. 2009 Oct;16(10):1485-92


The high osmotic response and cell wall integrity pathways cooperate to regulate transcriptional responses to zymolyase-induced cell wall stress in Saccharomyces cerevisiae.

García R, Rodríguez-Peña JM, Bermejo C, Nombela C, Arroyo J. J Biol Chem. 2009 Apr 17;284(16):10901-11


Genomics in the detection of damage in microbial systems: cell wall stress in yeast.

Arroyo J, Bermejo C, García R, Rodríguez-Peña JM. Clin Microbiol Infect. 2009 Jan;15 Suppl 1:44-6


Different modulation of the outputs of yeast MAPK-mediated pathways by distinct stimuli and isoforms of the dual-specificity phosphatase Msg5.

Marín MJ, Flández M, Bermejo C, Arroyo J, Martín H, Molina M. Mol Genet Genomics. 2009 Mar;281(3):345-59


Assembly of the yeast cell wall. Crh1p and Crh2p act as transglycosylases in vivo and in vitro.

Cabib E, Farkas V, Kosík O, Blanco N, Arroyo J, McPhie P. J Biol Chem. 2008 Oct 31;283(44):29859-72


The sequential activation of the yeast HOG and SLT2 pathways is required for cell survival to cell wall stress.

Bermejo C, Rodríguez E, García R, Rodríguez-Peña JM, Rodríguez de la Concepción ML, Rivas C, Arias P, Nombela C, Posas F, Arroyo J. Mol Biol Cell. 2008 Mar;19(3):1113-24


A yeast strain biosensor to detect cell wall-perturbing agents.

Rodriguez-Peña JM, Diez-Muñiz S, Nombela C, Arroyo J. J Biotechnol. 2008 Feb 1;133(3):311-7


Induction of paclitaxel resistance by the Kaposi's sarcoma-associated herpesvirus latent protein LANA2.

Muñoz-Fontela C, Marcos-Villar L, Hernandez F, Gallego P, Rodriguez E, Arroyo J, Gao SJ, Avila J, Rivas C. J Virol. 2008 Feb;82(3):1518-25


Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6) Glucan in the Saccharomyces cerevisiae cell wall.

E. Cabib, N. Blanco, C. Grau, J.M. Rodriguez-Peña and J. Arroyo. Molecular Microbiology. 2007. 63 (3): 921-935.


Latent protein LANA2 from Kaposi's sarcoma-associated herpesvirus interacts with 14-3-3 proteins and inhibits FOXO3a transcription factor.

C. Muñoz-Fontela, L. Marcos-Villar, P. Gallego, J. Arroyo, M. DaCosta, K.M. Pomeranz, E. Lam and C. Rivas. Journal Virolology. 2007. 81(3):1511-6


GAS2 and GAS4: a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae.

E. Ragni, A. Coluccio, E. Rolli, J.M. Rodríguez-Peña, G. Colasante, J. Arroyo, A. Neiman and L. Popolo.  Eukaryotic Cell. 2007. 6(2):302-16


Integrated proteomic and genomic strategies bring new insight into Candida albicans response upon macrophage interaction.

E. Fernández-Arenas, V. Cabezón, C. Bermejo, J. Arroyo, C. Nombela, R. Díez-Orejas, C. Gil.  Molecular and Cellular Proteomics. 2007. 6(3):460-78.


The GPI-anchored Gas and Crh families are fungal antigenes.

J. Arroyo , J. Sarfati , M.T. Baixench, E. Ragni,  M.V. Guillén , J.M. Rodriguez-Peña, L. Popolo and J.P. Latgé. Yeast. 2007 24(4):289-96.


Antiviral action of the tumor supresor ARF.

M.A García, M. Collado, C. Muñoz-Fontela, A. Matheau, L. Marcos-Villar, J. Arroyo, M. Esteban, M. Serrano and C. Rivas.  EMBO J. 2006. 25 (18): 4284-92.


The Cell-Wall Chip, a useful tool to study the regulation of cell wall construction in Saccharomyces cerevisiae.

J.M Rodríguez-Peña, R. Pérez-Díaz, S. Alvarez, C. Bermejo, R. García, C. Santiago, C. Nombela and J.  Arroyo.  Microbiology. 2005. 151: 2241-2249.


Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.

Nierman, W., Arnab, P., Anderson,M, Wortman, H., Kinm, S., Arroyo, J., .......and Denning, D.  Nature. 2005. 438 (7071):1151-1156.