"in vivo" tecniques

Determinations in rats and mice

• Blood Pressure and ECG
• Intraventricular pressure
• Cardiac function by NMR
• Adipose tissue distribution and metabolism
• Pharmacological treatments

"in vitro" techniques

• Large artery function by isometric tension recording
• Mesenteric bed function by perfusion pressure recording
• Wire myography
• Laser scanning confocal microscopy
• Cell culture: human and murine vascular smooth muscle cells, vascular fibroblasts, endothelial cells
• Oxidative stress parameter determination
• Molecular determinations: protein expression (Western blot) and mRNA (RT-qPCR), immunoprecipitation
• Biochemical studies: activity of metalloproteinases, renin-angiotensin system enymes, AMPK