Integridad celular

Cell Wall integrity

• Lavilla-García B, Fernández-Acero T, Molina M, Martín H. (2025). Integration of human ERKs and DUSPs into the yeast cell wall integrity pathway. Sci Rep. doi: 10.1038/s41598-025-33816-8
• González-Rubio G, Martín H, Molina M. (2023) The Mitogen-Activated Protein Kinase Slt2 Promotes Asymmetric Cell Cycle Arrest and Reduces TORC1-Sch9 Signaling in Yeast Lacking the Protein Phosphatase Ptc1. Microbiol Spectr. e0524922. doi: 10.1128/spectrum.05249-22.
•  de Oya IG, Jiménez-Gutiérrez E, Gaillard H, Molina M, Martín H, Wellinger RE. (2022). Manganese Stress Tolerance Depends on Yap1 and Stress-Activated MAP Kinases. Int J Mol Sci. 23:15706. doi: 10.3390/ijms232415706.
• Jiménez-Gutiérrez E, Fernández-Acero T, Alonso-Rodríguez E, Molina M, Martín H. (2022) Neomycin Interferes with Phosphatidylinositol-4,5-Bisphosphate at the Yeast Plasma Membrane and Activates the Cell Wall Integrity Pathway. Int J Mol Sci. ;23(19):11034. doi: 10.3390/ijms231911034.
• González-Rubio G, Sastre-Vergara L, Molina M, Martín H, Fernández-Acero T. (2022) Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity. J Fungi (Basel). 4;8(4):368. doi: 10.3390/jof8040368.

• Sellers-Moya Á, Nuévalos M, Molina M, Martín H. (2021). Clotrimazole-Induced Oxidative Stress Triggers Novel Yeast Pkc1-Independent Cell Wall Integrity MAPK Pathway Circuitry. 

  J Fungi (Basel). 7(8):647. doi: 10.3390/jof7080647.

• González-Rubio G, Sellers-Moya Á, Martín H, Molina M. (2021). A walk-through MAPK structure and functionality with the 30-year-old yeast MAPK Slt2. Int Microbiol. doi: 10.1007/s10123-021-00183-z.
• González-Rubio G, Sellers-Moya Á, Martín H, Molina M. (2021) Differential Role of Threonine and Tyrosine Phosphorylation in the Activation and Activity of the Yeast MAPK Slt2. Int J Mol Sci. 23;22(3):1110. doi: 10.3390/ijms22031110.PMID: 33498635 
• Jiménez-Gutiérrez E, Alegría-Carrasco E, Alonso-Rodríguez E, Fernández-Acero T, Molina M, Martín H. (2020). Rewiring the yeast cell wall integrity (CWI) pathway through a synthetic positive feedback circuit unveils a novel role for the MAPKKK Ssk2 in CWI pathway activation. FEBS J.  doi: 10.1111/febs.15288. PMID: 32150787.
• González-Rubio G, Fernández-Acero T, Martín H, Molina M. (2019) Mitogen-Activated Protein Kinase Phosphatases (MKPs) in Fungal Signaling: Conservation, Function, and Regulation.
   Int J Mol Sci. 20(7):1709. doi: 10.3390/ijms20071709. PMID: 30533419
• Jiménez-Gutiérrez E, Alegría-Carrasco E, Sellers-Moya Á,Molina M, Martín H. Not just the wall: the other ways to turn the yeast CWI pathway on. Int Microbiol. (2020) 23(1):107-119. doi: 10.1007/s10123-019-00092-2. PMID: 31342212.
• Alonso-Rodriguez, E., Fernandez-Pinar, P., Sacristan-Reviriego, A., Molina, M., and Martin, H. (2016) An analog-sensitive version of the protein kinase Slt2 allows identification of novel targets of the yeast cell wall integrity pathway. The Journal of biological chemistry 291: 5461-5472.
• Manzoor, S., Ugena, L., Tornero-Lopez, J., Martin, H., Molina, M., Camacho, J.J., and Caceres, J.O. (2016) Laser induced breakdown spectroscopy for the discrimination of Candida strains. Talanta 155: 101-106.
• Sacristan-Reviriego, A., Molina, M., and Martin, H. (2016) Methods to study protein tyrosine phosphatases acting on yeast MAPKs. Methods in molecular biology (Clifton, N.J.) 1447: 385-398.
• Tatjer, L., Sacristan-Reviriego, A., Casado, C., Gonzalez, A., Rodriguez-Porrata, B., Palacios, L., Canadell, D., Serra-Cardona, A., Martin, H., Molina, M., and Arino, J. (2016) Wide-ranging effects of the yeast Ptc1 protein phosphatase acting through the MAPK kinase Mkk1. Genetics 202: 141-156.
• Martin, H., Shales, M., Fernandez-Pinar, P., Wei, P., Molina, M., Fiedler, D., Shokat, K.M., Beltrao, P., Lim, W., and Krogan, N.J. (2015) Differential genetic interactions of yeast stress response MAPK pathways. Molecular systems biology 11: 800.
• Sacristan-Reviriego, A., Martin, H., and Molina, M. (2015) Identification of putative negative regulators of yeast signaling through a screening for protein phosphatases acting on cell wall integrity and mating MAPK pathways. Fungal genetics and biology : FG & B 77: 1-11.
• Llopis, S., Hernandez-Haro, C., Monteoliva, L., Querol, A., Molina, M., and Fernandez-Espinar, M.T. (2014) Pathogenic potential of Saccharomyces strains isolated from dietary supplements. PloS one 9: e98094
• Sacristán-Reviriego A, Madrid M, Cansado J, Martín H, Molina M. 2014. A conserved non-canonical docking mechanism regulates the binding of dual specificity phosphatases to cell integrity mitogen-activated protein kinases (MAPKs) in budding and fission yeasts. PLoS One. 2014 Jan 22;9(1):e85390.
• Mascaraque V, Hernáez ML, Jiménez-Sánchez M, Hansen R, Gil C, Martín H, Cid VJ, Molina M. 2013. Phosphoproteomic analysis of protein kinase C signaling in Saccharomyces cerevisiae reveals Slt2 mitogen-activated protein kinase (MAPK)-dependent phosphorylation of eisosome core components. Mol Cell Proteomics. 12:557-74.
  
• Palacios L, Dickinson RJ, Sacristan-Reviriego A, Didmon MP, Marin MJ, Martin H, Keyse SM, Molina M. 2011. Distinct docking mechanisms mediate interactions between the Msg5 phosphatase and mating or cell integrity MAPKs in S. cerevisiae. J Biol Chem. 286:42037-42050. DOI
• Romá-Mateo C, Sacristán-Reviriego A, Beresford NJ, Caparrós-Martín JA, Culiáñez-Macià FA, Martín H, Molina M, Tabernero L, Pulido R. 2011. Phylogenetic and genetic linkage between novel atypical dual-specificity phosphatases from non-metazoan organisms. Mol Genet Genomics 285:341-54.
• Insenser MR, Hernáez ML, Nombela C, Molina M, Molero G, Gil C. 2010. Gel and gel-free proteomics to identify Saccharomyces cerevisiae cell surface proteins. J Proteomics 73:1183-95.
• de Llanos R, Hernández-Haro C, Barrio E, Querol A, Fernández-Espinar MT,Molina M. 2010. Differences in activation of MAP kinases and variability in the polyglutamine tract of Slt2 in clinical and non-clinical isolates of Saccharomyces cerevisiae. Yeast. 27:549-561.
• Molina M, Cid VJ, Martín H. 2010. Fine regulation of Saccharomyces cerevisiae MAPK pathways by post-translational modifications. Yeast 27:503–511
• Marín MJ, Flández M, Bermejo C, Arroyo J, Martín H, Molina M. 2009. Different modulation of the outputs of yeast MAPK-mediated pathways by distinct stimuli and isoforms of the dual-specificity phosphatase Msg5. Mol Genet Genomics. 281:345-59.
• Jiménez-Sánchez M, Cid VJ, Molina M. 2007. Retrophosphorylation of Mkk1 and Mkk2 MAPKKs by the Slt2 MAPK in the yeast cell integrity pathway. J Biol Chem. 282:31174-85.
• Kim KY, Cosano IC, Levin DE, Molina M, Martín H. 2007. Dissecting the transcriptional activation function of the cell wall integrity MAP kinase. Yeast. 24:335-42.
• Martín H, Flández M, Nombela C, Molina M. 2005. Protein phosphatases in MAPK signalling: we keep learning from yeast. Mol Microbiol. 58:6-16.
• Flández M, Cosano IC, Nombela C, Martín H, Molina M. 2004. Reciprocal regulation between Slt2 MAPK and isoforms of Msg5 dual-specificity protein phosphatase modulates the yeast cell integrity pathway. J Biol Chem. 279:11027-34.

Levaduras humanizadas

Humanized Yeasts

• López-Montesino S, Coronas-Serna JM,  Martín H, Molina M, Cid VJ (2026) Distinct Domains Contribute to the Subcellular Localization of Human cGAS in Yeast. Biomolecules 2026, 16(2), 259; doi.org/10.3390/biom16020259

• Del Val E, Fernández-Vega A, Molina M, Cid VJ. (2025). MyD88 polymerization and association to cellular membranes in a yeast heterologous model. Cell Mol Life Sci. 82(1):288. doi: 10.1007/s00018-025-05827-1.
• Barbero-Úriz Ó, Valenti M, Molina M, Fernández-Acero T, Cid VJ.  (2025). Modeling Necroptotic and Pyroptotic Signaling in Saccharomyces cerevisiae.
  Biomolecules. 15(4):530. doi: 10.3390/biom15040530.
• Valenti M, Molina M, Cid VJ. (2023). Human gasdermin D and MLKL disrupt mitochondria, endocytic traffic and TORC1 signalling in budding yeast. Open Biol.13: 220366. doi.org/10.1098/rsob.220366
• Torices L, Mingo J, Rodríguez-Escudero I, Fernández-Acero T, Luna S, Nunes-Xavier CE, López JI, Mercadillo F, Currás M, Urioste M, Molina M, Cid VJ, Pulido R. (2022) Functional analysis of PTEN variants of unknown significance from PHTS patients unveils complex patterns of PTEN biological activity in disease. Eur J Hum Genet. doi: 10.1038/s41431-022-01265-w.
• Coronas-Serna, JM, del Val E, Kagan JC, Molina M, Cid VJ (2021). 

  Heterologous Expression and Assembly of Human TLR Signaling Components inSaccharomyces cerevisiae. Biomolecules, 11(11), 1737; doi.org/10.3390/biom11111737

• Valenti M, Molina M, Cid VJ. (2021). Heterologous Expression and Auto-Activation of Human Pro-Inflammatory Caspase-1 in Saccharomyces cerevisiae and Comparison to Caspase-8. Front Immunol. 12:668602 doi: 10.3389/fimmu.2021.668602.

• Luna S, Torices L, Mingo J, Amo L, Rodríguez-Escudero I, Ruiz-Ibarlucea P, Erramuzpe A, Cortés JM, Tejada MI, Molina M, Nunes-Xavier CE, López JI, Cid VJ, Pulido R. (2021). 

  A global analysis of the reconstitution of PTEN function by translational readthrough of PTEN pathogenic premature termination codons. 

  Hum Mutat. 42(5):551-566. doi: 10.1002/humu.24186.

• Fernández-Acero T, Bertalmio E, Luna S, Mingo J, Bravo-Plaza I, Rodríguez-Escudero I, Molina M, Pulido R,Cid VJ. (2019) Expression of Human PTEN-L in a Yeast Heterologous Model Unveils Specific N-Terminal Motifs Controlling PTEN-L Subcellular Localization and Function. Cells. 8(12):1512. doi: 10.3390/cells8121512
• Coronas-Serna JM, Valenti M, Del Val E, Fernández-Acero T, Rodríguez-Escudero I, Mingo J, Luna S, Torices L, Pulido R,Molina M,Cid VJ. (2020) Modeling human disease in yeast: recreating the PI3K-PTEN-Akt signaling pathway in Saccharomyces cerevisiae. Int Microbiol.  23(1):75-87. doi: 10.1007/s10123-019-00082-4. PMID: 31218536.
• Rodríguez-Escudero I, Fernández-Acero T, Cid VJ, Molina M. Heterologous mammalian Akt disrupts plasma membrane homeostasis by taking over TORC2 signaling in Saccharomyces cerevisiae. 2018. Sci Rep. 8(1): Article 7732. DOIi: 10.1038/s41598-018-25717-w.
• Mingo J, Rodríguez-Escudero I, Luna S, Fernández-Acero T, Amo L, Jonasson AR, Zori RT, López JI, Molina M, Cid VJ, Pulido R. A pathogenic role for germline PTEN variants which accumulate into the nucleus. 2018. Eur J Hum Genet. DOI: 10.1038/s41431-018-0155-x.
• Oliver MD, Fernández-Acero T, Luna S, Rodríguez-Escudero I, Molina M, Pulido R, Cid VJ. Insights into the pathological mechanisms of p85α mutations using a yeast-based phosphatidylinositol 3-kinase model. 2017. Biosci Rep. Mar 15;37(2). doi: 10.1042/BSR20160258.
• Fernandez-Acero, T., Rodriguez-Escudero, I., Molina, M., and Cid, V.J. (2015) The yeast cell wall integrity pathway signals from recycling endosomes upon elimination of phosphatidylinositol (4,5)-bisphosphate by mammalian phosphatidylinositol 3-kinase. Cellular signalling 27: 2272-2284.
• Gil, A., Rodriguez-Escudero, I., Stumpf, M., Molina, M., Cid, V.J., and Pulido, R. (2015) A functional dissection of PTEN N-terminus: implications in PTEN subcellular targeting and tumor suppressor activity. PloS one 10: e0119287.
• Rodriguez-Escudero, I., Fernandez-Acero, T., Bravo, I., Leslie, N.R., Pulido, R., Molina, M., and Cid, V.J. (2015) Yeast-based methods to assess PTEN phosphoinositide phosphatase activity in vivo. Methods (San Diego, Calif.) 77-78: 172-179.
• Fernández-Acero T, Rodríguez-Escudero I, Vicente F, Monteiro MA, Tormo JR,  Cantizani J, Molina M, Cid VJ. 2012. A yeast-based in vivo bioassay to screen for class I phosphatidylinositol 3-kinase specific inhibitors. Journal of Biomolecular Screening 17:1018-29.
• Rodríguez-Escudero I, Oliver MD, Andrés-Pons A, Molina M, Cid VJ, Pulido R. 2011. A comprehensive functional analysis of PTEN mutations: implications in tumor- and autism-related syndromes. Hum Mol Genet. 20:4132-42.
• Rodriguez-Escudero I, Andres-Pons A, Pulido R, Molina M, Cid VJ. 2009. PI3K-dependent activation of mammalian PKB/Akt in S. cerevisiae: an in vivo model for the functional study of Akt mutations. J Biol Chem. 284:13373-13383.
• Cid VJ, Rodríguez-Escudero I, Andrés-Pons A, Romá-Mateo C, Gil A, den Hertog J, Molina M, Pulido R. 2008. Assessment of PTEN tumor suppressor activity in nonmammalian models: the year of the yeast. Oncogene. 27:5431-42.
• Andrés-Pons A, Rodríguez-Escudero I, Gil A, Blanco A, Vega A, Molina M, Pulido R, Cid VJ. 2007. In vivo functional analysis of the counterbalance of hyperactive phosphatidylinositol 3-kinase p110 catalytic oncoproteins by the tumor suppressor PTEN. Cancer Res. 67:9731-9.
• Rodríguez-Escudero I, Roelants FM, Thorner J, Nombela C, Molina M, Cid VJ. 2005. Reconstitution of the mammalian PI3K/PTEN/Akt pathway in yeast. Biochem J. 390:613-23.

Virulencia de patógenos

Pathogen virulence factors

• Sá-Pessoa J, López-Montesino S, Przybyszewska K, Rodríguez-Escudero I, Marshall H, Ova A, Schroeder GN, Barabas P, Molina M, Curtis T, Cid VJ, Bengoechea JA. (2023). A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infection. Nat Commun.  14(1):871. doi: 10.1038/s41467-023-36629-3. PMID: 36797302.
• Coronas-Serna JM, Louche A, Rodríguez-Escudero M, Roussin M, Imbert PRC, Rodríguez-Escudero I, Terradot L,Molina M, Gorvel JP, Cid VJ, Salcedo SP. (2020) The TIR-domain containing effectors BtpA and BtpB from Brucella abortus impact NAD metabolism. PLoS Pathogens. 16(4):e1007979. doi: 10.1371/journal.ppat.1007979. PMID: 32298382.
• Storey D, McNally A, Åstrand M, Sa-Pessoa Graca Santos J, Rodriguez-Escudero I, Elmore B, Palacios L, Marshall H, Hobley L,Molina M, Cid VJ, Salminen TA, Bengoechea JA.  (2020)  Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog. 16(3):e1007969. doi: 10.1371/journal.ppat.1007969. PMID: 32191774.
• Domingues, L., Ismail, A., Charro, N., Rodriguez-Escudero, I., Holden, D.W., Molina, M., Cid, V.J., and Mota, L.J. (2016) The Salmonella effector SteA binds phosphatidylinositol 4-phosphate for subcellular targeting within host cells. Cellular Microbiology 18: 949-969.
• Rodriguez-Escudero, M., Cid, V.J., Molina, M., Schulze-Luehrmann, J., Luhrmann, A., and Rodriguez-Escudero, I. (2016) Studying Coxiella burnetii Type IV substrates in the yeast Saccharomyces cerevisiae: Focus on subcellular localization and protein aggregation. PloS One 11: e0148032.
• Fernandez-Piñar P, Alemán A, Sondek J, Dohlman HG, Molina M, Martín H. 2012. The Salmonella Typhimurium effector SteC inhibits Cdc42-mediated signaling through binding to the exchange factor Cdc24 in Saccharomyces cerevisiae. Mol. Biol. Cell. 23:4430-43.
• Rodríguez-Escudero I, Ferrer N, Rotger R, Cid VJ, Molina M. 2011. Interaction of the Salmonella Typhimurium effector protein SopB with host cell Cdc42 is involved in intracellular replication. Mol. Microbiol. 8:1220-24.
• Cid VJ, Kauffmann E, Molina M. 2010. Reverse protein arrays applied to host-pathogen interaction studies. Met. Mol. Biol. Totowa, NJ: Humana Press. 723:37-55.
• Alemán A, Fernández-Piñar P, Pérez-Núñez D, Rotger R, Martín H, Molina M. 2009. A yeast-based genetic screen for identification of pathogenic Salmonella proteins. FEMS Microbiol. Lett. 296:167-177.
• Molero C, Rodríguez-Escudero I, Alemán A, Rotger R, Molina M, Cid VJ. 2009. Addressing the effects of Salmonella internalization in host cell signaling on a reverse-phase protein array. Proteomics 9:1-14.
• Rodríguez-Escudero I, Rotger R, Cid VJ, Molina M. 2006. Inhibition of Cdc42-dependent signalling in Saccharomyces cerevisiae by phosphatase-dead SigD/SopB from SalmonellaTyphimurium. Microbiology-SGM 152:3437-52.
• Alemán A, Rodríguez-Escudero I, Mallo GV, Cid VJ, Molina M, Rotger R. 2005. The amino-terminal non-catalytic region ofSalmonella typhimurium SigD affects actin organization in yeast and mammalian cells. Cell Microbiol. 7:1432-46.
• Rodríguez-Escudero I, Hardwidge PR, Nombela C, Cid VJ, Finlay BB, Molina M. 2005. Enteropathogenic Escherichia coli type III effectors alter cytoskeletal function and signalling inSaccharomyces cerevisiae. Microbiology. 151:2933-45.
• Hardwidge PR, Deng W, Vallance BA, Rodriguez-Escudero I, Cid VJ, Molina M, Finlay BB. 2005. Modulation of host cytoskeleton function by the enteropathogenic Escherichia coli and Citrobacter rodentium effector protein EspG. Infect. Immun. 73:2586-94.
• Rodríguez-Pachón JM, Martín H, North G, Rotger R, Nombela C, Molina M. 2002. A novel connection between the yeast Cdc42 GTPase and the Slt2-mediated cell integrity pathway identified through the effect of secreted Salmonella GTPase modulators. J. Biol. Chem. 277:27094-102.