| Description |
HiCSA has a wide range of collaboration with both national and international institutions and bodies. Nationally, HiCSA has worked with a variety of non-profits (university Cinémathèque, Réforme Humanisme, Renaissance, Les Trois Lumières and the French association of sociology) and learned societies (such as the Société Française d’Étude du Seizième Siècle, and the Société Française de Photographie and its journal Études Photographiques). HiCSA also has relations with many national and international museums and committees of the French Ministry of Culture. Close collaborations with this institutions give HiCSA possibility to transfer knowledge through joint projects: exhibitions, media productions, joint public talks, etc. |
| Advantages |
For application, the first step is the collection and preparation of the samples, considering the conditions of collection, storage and transport. Usually, the microbiological samples will be taken from locations evaluated by a specialist with sterile paper points. These paper points will be deposited in a vial containing a sterile reduced transport fluid (RTF), preserving the anaerobiosis. After a maximum period of 24 hours from collection until receipt at the laboratory, samples will be dispersed, and we will proceed to the extraction and purification of deoxyribonucleic acid (DNA), to perform then the specific analysis by PCR technique. Different DNA-based protocols will be used, based on particular chemical, enzymatic and / or physical agents to release the genetic material depending on the characteristics of the samples. It continues with the purification of the extracted material, and then it will be subjected to amplification techniques, either by the technique of qualitative PCR, using specific primers for selected bacteria, or by quantitative PCR with incorporation plus fluorescent label for quantitation of these bacteria in samples. These techniques allow us to detect and quantify, specifically and with high sensitivity, possible periodonto-pathogens in oral samples.
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