Grupos de investigación

  1. Portada
  2. Investigación
  3. Proteínas tóxicas y proteínas virales
  4. Publicaciones recientes

Publicaciones recientes

2024

  •  A. Damsbo, C. Rimbault, N.J. Burlet, A. Vlamynck, I. Bisbo, S.B. Belfakir, A.H. Laustsen, E. Rivera-de-Torre (2024) A comparative study of the performance of E. coli and K. phaffii for expressing α-cobratoxin. Toxicon 239:107613. doi: 10.1016/j.toxicon.2024.107613
  •  C. García-Montoya, S. García-Linares, D. Heras-Márquez, M. Majnik, D. Laxalde-Fernández, R. Amigot-Sánchez, Á. Martínez-del-Pozo, J. Palacios-Ortega (2024) The interaction of the ribotoxin α-sarcin with complex model lipid vesicles. Arch Biochem Biophys 751:109836. doi: 10.1016/j.abb.2023.109836

2023

  •  A. Robles-Martín, R. Amigot-Sánchez, L. Fernandez-Lopez, J.L. Gonzalez-Alfonso, S. Roda, V. Alcolea-Rodriguez, D. Heras-Márquez, D. Almendral, C. Coscolín, F.J. Plou, R. Portela, M.A. Bañares, Á. Martínez-del-Pozo, S. García-Linares, M. Ferrer, V. Guallar (2023) Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores. Nature Catalysis. doi: 10.1038/s41929-023-01048-6
  •  C. García-Montoya, D. Heras-Márquez, R. Amigot-Sánchez, S. García-Linares, A. Martínez-Del-Pozo, J. Palacios-Ortega (2023) Sticholysin recognition of ceramide-phosphoethanolamine. Arch Biochem Biophys 742:109623. doi: 10.1016/j.abb.2023.109623.

2022

  •  J. Palacios-Ortega, R. Amigot-Sánchez, C. García-Montoya, A. Gorše, D. Heras-Márquez, S. García-Linares, Á. Martínez-Del-Pozo, J.P. Slotte (2022) Determination of the boundary lipids of sticholysins using tryptophan quenching. Scientific Reports 12 (1), 17328. doi: 10.1038/s41598-022-21750-y
  •  J. Palacios-Ortega, D. Heras-Márquez, R. Amigot-Sánchez, C. García-Montoya, C. Torrijos, D. Laxalde, J.G. Gavilanes, S. García-Linares, Á. Martínez-Del-Pozo (2022) Sea Anemones, Actinoporins, and Cholesterol. International Journal of Molecular Science 23 (15), 8771. doi: 10.3390/ijms23158771.
  •  S. García-Linares, R. Amigot-Sánchez, C. García-Montoya, D. Heras-Márquez, C. Alfonso, J.R. Luque-Ortega, J.G. Gavilanes, Á. Martínez-del-Pozo, J. Palacios-Ortega (2022) Sticholysin I–II oligomerization in the absence of membranes. FEBS Letters. 596 (8), 1029-1036. doi: 10.1002/1873-3468.14326.
  •  P. Huang, B.M. Wierbowski, T.Lian, C. Chan, S. García-Linares, J. Jiang, A. Salic (2022) Structural basis for catalyzed assembly of the Sonic hedgehog–Patched1 signaling complex. Developmental Cell. 57: 1-16. doi: 10.1016/j.devcel.2022.02.008
  •  E. Rivera-de-Torre, C. Rimbault, T.P. Jenkins, C.V. Sørensen, A. Damsbo, N.J. Saez, Y. Duhoo, C.M. Hackney, L. Ellgaard, A.H. Laustsen (2022) Strategies for heterologous expression, synthesis, and purification of animal venom toxins. Frontiers in Bioengineering and Biotechnology. 9:811905. doi: 10.3389/fbioe.2021.811905.

 

2021

  •  J. Palacios-Ortega, S. García-Linares, E. Rivera-de-Torre, Diego Heras-Márquez, J.G. Gavilanes, J.P. Slotte y A Martínez-del-Pozo (2021) Structural Foundations of Sticholysin Functionality. BBA-Proteins and Proteomics. 1869 (10),140696. doi: 10.1016/j.bbapap.2021.140696.
  •  J. Palacios-Ortega, E. Rivera-de-Torre, S. García-Linares, J.G. Gavilanes, A. Martínez-del-Pozo y J.P. Slotte (2021) Oligomerization of sticholysins from Förster resonance energy transfer. Biochemistry 60 (4), 314-323. doi: 10.1021/acs.biochem.0c00840. Este artículo fue seleccionado por la SEBBM como “Artículo del mes” (Noviembre 2021).
  •  J. Palacios-Ortega, E. Rivera-de-Torre, J.G. Gavilanes, J.P. Slotte, A. Martínez-del-Pozo y S. García-Linares (2021) Biophysical approaches to study actinoporin-lipid interactions. Methods in Enzymology 690, 307-340. https://doi.org/10.1016/bs.mie.2020.12.017.

 

2020

  •  E. Rivera-de-Torre, J. Palacios-Ortega, J.P. Slotte, J.G. Gavilanes, A. Martínez-del-Pozo y S. García-Linares (2020) Functional and structural variation among sticholysins, pore-forming proteins from the sea anemone Stichodactyla helianthus. International Journal of Molecular Sciences 21, 8915. doi:10.3390/ijms21238915.
  •  R. Lázaro-Gorines, J.C. López-Rodríguez, S. Benedé, M. González, C. Mayorga, L. Vogel, A. Martínez-del-Pozo, J. Lacadena y M. Villalba (2020) Der p 1-based immunotoxin as potential tool for the treatment of dust mite respiratory allergy. Scientific Reports 10 (1), 12255. doi: 10.1038/s41598-020-69166-w.
  •  E. Rivera-de-Torre, J. Palacios-Ortega, J.E. Garb, J.P. Slotte, J.G. Gavilanes y A Martínez-del-Pozo (2020) Structural and functional characterization of Sticholysin III: A newly discovered actinoporin within the venom of the sea anemone Stichodactyla helianthus. Archives of Biochemistry and Biophysics 689, 108435. doi: 10.1016/j.abb.2020.108435.
  •  P. Hernansanz-Agustín, C. Choya-Foces, S. Carregal-Romero, E. Ramos, T. Oliva, T. Villa-Piña, L. Moreno, A. Izquierdo-Álvarez, J.D. Cabrera-García, A. Cortés, A.V. Lechuga-Vieco, P. Jadiya, E. Navarro, E. Parada, A. Palomino-Antolín, D. Tello, R. Acín-Pérez, J.C. Rodríguez-Aguilera, P. Navas, Á. Cogolludo, I. López-Montero, A. Martínez-del-Pozo, J. Egea, M.G. López, J.W. Elrod, J. Ruiz-Cabello, A. Bogdanova, J.A. Enríquez y A. Martínez-Ruiz (2020) Na+ controls hypoxic signalling by the mitochondrial respiratory chain. Nature 586, 287-291. doi: 10.1038/s41586-020-2551-y.
  • Á. Partida-Hanon, M. Maestro-López, S. Vitale, D. Turrà, A. Di Pietro, A. Martínez-del Pozo y M. Bruix (2020) Structure of fungal α mating pheromone in membrane mimetics suggests a possible role for regulation at the water-membrane interface Frontiers in Microbiology 11, 1090. doi: 10.3389/fmicb.2020.01090.
  •  M. Olombrada, C. Peña, O. Rodríguez-Galán, P. Nerurkar, M. Altvater, J.G. Gavilanes, A. Martínez-del-Pozo, J. de la Cruz, L. García-Ortega y V. G. Panse (2020) The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes. Nucleic Acids Research 48 (11), 6210-6222. doi: 10.1093/nar/gkaa315.
  •  J. Palacios-Ortega, E. Rivera-de-Torre, J.G. Gavilanes, J.P. Slotte y A. Martínez-del-Pozo (2020) Evaluation of different approaches used to study membrane permeabilization by actinoporins on model lipid vesicles. BBA - Biomembranes 1862 (9), 183311. doi: 10.1016/j.bbamem.2020.183311. Este artículo fue seleccionado por la SEBBM como “Artículo del mes” (Agosto 2020).

 

2019

  •  R. Lázaro-Gorines, J. Ruiz de la Herrán, R. Navarro, L. Sanz, L. Álvarez-Vallina, A. Martínez-del-Pozo, J.G. Gavilanes, y J. Lacadena (2019) A novel carcinoembryonic antigen (cea)-targeted trimeric immunotoxin shows significantly enhanced antitumor activity in human colorectal cancer xenografts. Scientific Reports 9, 11680. https://doi.org/10.1038/s41598-019-48285-z.
  •  E. Rivera-de-Torre, J. Palacios-Ortega, J.G. Gavilanes, A. Martínez-del-Pozo y S. García-Linares (2019) Pore-Forming-Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools. Toxins 11, 370. https://doi.org/10.3390/toxins11060370.
  •  J. Palacios-Ortega, S. García-Linares, E. Rivera-de-Torre, J.G. Gavilanes, A Martínez-del-Pozo y J.P. Slotte (2019) Sticholysin, Sphingomyelin, and Cholesterol: A Closer Look at a Tripartite Interaction. Biophysical Journal 116, 2253-2265. https://doi.org/10.1016/j.bpj.2019.05.010. Este artículo fue seleccionado por la SEBBM como “Artículo del mes” (agosto).

 

2018

  •  B. Patiño, C. Vázquez, J.M. Manning, M.I.G. Roncero, D. Córdoba-Cañero, A. Di Pietro y A. Martínez-del-Pozo (2018) Characterization of a new cysteine-rich antifungal protein from Fusarium graminearum with potential application for corn growth and storage. International Journal of Food Microbiology 283, 45-51. https://doi.org/10.1016/j.ijfoodmicro.2018.06.017.
  •  E. Rivera-de-Torre, A. Martínez-del-Pozo, y J.E. Garb, J.E. (2018) Stichodactyla helianthus’ de novo transcriptome assembly: discovery of a new actinoporin isoform. Toxicon 150, 105-114. https://doi.org/10.1016/j.toxicon.2018.05.014.
  •  L. García-Ortega, J. Palacios-Ortega y A. Martínez-del-Pozo (2018) Fungal Ribotoxins. Encyclopedia of Life Sciences Febrero, 1-9. John Wiley & Sons, Ltd: Chichester. DOI: 10.1002/9780470015902.a0027741.

  •  J. Gómez-Gutiérrez, M. Rodríguez-Rodríguez, F. Gavilanes, y B. Yélamos (2018) Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2Ectodomain Produced in a Baculovirus Expression System. Bio-Protocol 8, e3030. doi: 10.21769/BioProtoc.3030

  •  

    M. Rodríguez-Rodríguez, D. Tello, J. Gómez-Gutiérrez, D.L. Peterson, F. Gavilanes, y B. Yélamos (2018) Fusogenic properties of the Ectodomain of HCV E2 envelope protein. BBA - Biomembranes 1860, 728-36. doi: 10.1016/j.bbamem.2017.12.017

  •  

    B. Yélamos, M. Rodríguez-Rodríguez, F. Gavilanes, y J. Gómez-Gutiérrez (2018) In vitro Membrane Interaction and Liposome Fusion Assays Using Recombinant Hepatitis C Virus Envelope Protein E2. Bio- Protocol8, e3108. doi: 10.21769/BioProtoc.3108

 

2017

  •  E. Rivera-de-Torre, J. Palacios-Ortega, S. García-Linares, J.G. Gavilanes y A. Martínez-del-Pozo (2017) One single salt bridge explains the different cytolytic activities shown by actinoporins sticholysin I and II from the venom of S. helianthus. Archives of Biochemistry and Biophysics 636, 79-89. DOI: 10.1016/j.abb.2017.11.005.
  •  J. Palacios-Ortega, S. García-Linares, E. Rivera-de-Torre, J.G. Gavilanes, A. Martínez-del-Pozo y J.P. Slotte (2017) Differential effect of bilayer thickness on sticholysin activity. Langmuir 33, 11018-11027. DOI: 10.1021/acs.langmuir.7b01765.
  •  S. García-Linares, E. Rivera-de-Torre, J. Palacios-Ortega, J.G. Gavilanes y A. Martínez-del-Pozo (2017) The metamorphic transformation of a water-soluble monomeric protein into an oligomeric transmembrane pore. Advances in Biomembranes and Lipid Self-Assembly 26, 51-97.
  •  M. Maestro-López, M. Olombrada, L. García-Ortega, D. Serrano-González, J. Lacadena, M. Oñaderra, J.G. Gavilanes y A. Martínez-del-Pozo (2017) “Minimized natural versions of fungal ribotoxins show improved active site plasticity”. Archives of Biochemistry and Biophysics 619, 45-53. DOI 10.1016/j.abb.2017.03.002.
  •  M. Olombrada, R. Lázaro-Gorines, J.C. López-Rodríguez, A. Martínez-del-Pozo, M. Oñaderra, M. Maestro-López, J. Lacadena, J.G. Gavilanes y L. García-Ortega (2017) Fungal ribotoxins: A Review of Potential Biotechnological Applications. Toxins 9 (2), E71. doi: 10.3390/toxins9020071.
  •  S. Vitale, A. Partida-Hanon, S. Serrano, A. Martínez-del-Pozo, A. Di Pietro, D. Turrà y M. Bruix (2017) “Structure-activity relationship of α mating pheromone from the fungal pathogen Fusarium oxysporum”. The Journal of Biological Chemistry 292 (9), 3591-3602. doi: 10.1074/jbc.M116.766311.
  •  M. Olombrada, P. Medina, F. Budia, J.G. Gavilanes, A. Martínez-del-Pozo y L. García-Ortega (2017) Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin. Biological Chemistry 398 (1), 135-142. doi: 10.1515/hsz-2016-0119.

 

2016

  •  S. García–Linares, E. Rivera–de–Torre, K. Morante, K. Tsumoto, J.M.M. Caaveiro, J.G. Gavilanes, J.P. Slotte, A. Martínez-del-Pozo (2016) Differential effect of membrane composition on the pore–forming behavior of four different sea anemone actinoporins. Biochemistry 55 (48) 6630-6641. DOI: 10.1021/acs.biochem.6b01007.
  •  S. García-Linares, T. Maula, E. Rivera-de-Torre, J.G. Gavilanes, J.P. Slotte y A. Martínez-del-Pozo (2016) Role of the tryptophan residues in the specific interaction with biological membranes of the sea anemone actinoporin StnII from Stichodactyla helianthus. Biochemistry 55 (46), 6406−6420. DOI: 10.1021/acs.biochem.6b00935.
  •  E. Rivera-de-Torre, S. García-Linares, J. Alegre-Cebollada, J Lacadena, J.G. Gavilanes y A. Martínez-del-Pozo (2016) Synergistic action of actinoporin isoforms from the same sea anemone species assembled into functionally active heteropores. The Journal of Biological Chemistry 291 (27), 14109–14119. doi:10.1074/jbc.M115.710491. Este artículo fue elegido como artículo del mes por la Sociedad de Biofísica de España.
  •  J. Palacios-Ortega, S. García-Linares, M. Åstrand, Md. A. Al Sazzad, J.G. Gavilanes, A. Martínez-del-Pozo y J.P. Slotte (2016) Regulation of Sticholysin II-induced Pore Formation by Lipid Bilayer Composition, Phase State, and Interfacial Properties. Langmuir 32 (14), 3476-3484.
  •  S. García-Linares, J. Palacios-Ortega, T. Yasuda, M. Åström, J.G. Gavilanes, A. Martínez-del-Pozo y J.P. Slotte (2016) Toxin-induced pore formation is hindered by intermolecular hydrogen bonding in sphingomyelin bilayers. Biochimica et Biophysica Acta–Biomembranes 1858 (6), 1189-1195. doi:10.1016/j.bbamem.2016.03.013.
  •  M. Olombrada; L. García-Ortega, J. Lacadena, M. Oñaderra, J.G. Gavilanes y A. Martínez-del-Pozo (2016) Involvement of loop 5 lysine residues and the N-terminal β-hairpin of the ribotoxin hirsutellin A on its insecticidal activity. Biological Chemistry 397 (2), 135-145. DOI: 10.1515/hsz-2015-0261.
  •  

    J. Buendia, F. García, B. Yélamos, y L. Sánchez (2016) Transfer and amplification of chirality in Phe-based C3-symmetric non-ionic amphiphiles. Chemical Communications (Cambridge) 52, 8830-8833. doi: 10.1039/c6cc04273b

  •  L. Lombana, S. Ortega-Atienza, J. Gómez-Gutiérrez, B. Yélamos, D.L. Peterson, y F. Gavilanes (2016) The deletion of residues 268-292 of E1 impairs the ability of HCV envelope proteins to induce pore formation. Virus Research 217, 63-70. doi: 10.1016/j.virusres.2016.02.009