Gene and Protein expression
Quantitative polymerase chain reaction (qPCR)
Quantitative polymerase chain reaction (qPCR) is used to amplify and quantify a particular portion of DNA. In most cases, the first thing to do is obtaining mRNA from cells or tissues. mRNA is synthetized in cells as the first step in the expression of some protein. This mRNA can be convert to cDNA and then we can perform qPCR. In this sense, knowing the sequence of a gene, which encodes a particular protein through mRNA, we are able to know the percentage of its expression (quantity of DNA is proportional to its amplification).
Western blot is commonly used to identify specific proteins in a complex mixture obtain from cells or tissue using antibodies for them. Furthermore, we are able to quantify them relatively, which is very useful to study alterations in biochemical cascades in diseases due to changes in its normal quantity.
Transcriptomics We useRNA-Seq with next-generation sequencing (NGS) to analyze simultaneously the expression of thousands of mRNAs in biological samples.