Cell culture

Our group has large experience in the culture of different cell lines (pulmonary artery smooth muscle cells, pulmonary artery endotelial cells, human umbilical vein endotelial cells, alveolar epithelial cells, mesenchymal stem cells...).


Cell proliferation is analysed by cell count and colorimetric methods as BrdU assay (based on the measurement of the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) incorporation during DNA synthesis) or MTT assay (the thiazolyl blue tetrazolium bromide (MTT) produces a yellowish solution that is converted to dark blue MTT formazan by mitochondrial dehydrogenases of living cells).

Apoptosis studies are performed analysing the cleaved caspase-3 expression and TUNEL /propidium iodide staining using standard kits.


Scratch-wound healing assay